SPADA Book - April 11, 2017
Burkholderia mallei/pseudomallei
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Bacillus anthracis Inclusivity Panel – Tier 1
A4.1.1 Bacillus anthracis. If any listed strain is not available, an alternate strain shall be selected from the same VNTR group. If an alternate strain from the same VNTR group is not available, a strain from the same genotype shall be selected. If an alternate strain from the same genotype is not available, a strain different than those listed below shall be selected if available;
Tier 1 Bacillus anthracis Inclusivity Panel Species Strain
Notes
Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis A Bacillus anthracis Bacillus anthracis Bacillus anthracis B Bacillus anthracis Bacillus anthracis B Bacillus anthracis
Ames
pXO1+, pXO2+; VNTR group A3b; genotype 62 pXO1+, pXO2+, VNTR group B1; genotype 82 pXO1+, pXO2+; VNTR group A1a; genotype 7 pXO1+, pXO2+; VNTR group A3c; genotype 67 pXO1+, pXO2+; VNTR group A3d; genotype 68 pXO1+, pXO2+; VNTR group A2; genotype 29 pXO1-, pXO2+; VNTR group A1a; genotype 8 pXO1+, pXO2+; VNTR group B2; genotype 80 pXO1+, pXO2+; VNTR group A4; genotype 69 pXO1+, pXO2-; VNTR group A3b; genotype 59, 61 pXO1+, pXO2+; VNTR group A1b; genotype 23 pXO1+, pXO2-; VNTR group A3A; genotype 45
BA1035 BA0018
K3
Ohio ACB
PAK-1 Pasteur
RA3
SK-102
Sterne
Turkey 32
V770-NP-1R Vollum 1B
pXO1+, pXO2+; VNTR group A4; genotype 77 Adapted from Official Methods of Analysis of AOAC INTERNATIONAL (2016) 20th Ed., AOAC INTERNATIONAL, Rockville, MD, USA, AOAC SMPR 2010.003, www.eoma.aoac.org,and Bacillus anthracis 2016.006 - Journal of AOAC International, Volume 99, Number 4, July-August 2016, pp. 1084-1089. https://doi.org/10.5740/jaoacint.SMPR2016.006. A This strain should not be included in the inclusivity panel if the product being tested only utilizes a pXO1 assay, as this strain is expected to be pXO1 negative. B These two strains should not be included in the inclusivity panel if the product being tested only utilizes a pXO2 assay, as these strains are expected to be pXO2 negative.
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