SPADA Book - April 11, 2017
Bacillus anthracis Inclusivity Panel – Tier 2
A4.2.1 Bacillus anthracis . If any listed strain is not available, an alternate strain shall be selected from the same VNTR group. If an alternate strain from the same VNTR group is not available, a strain from the same genotype shall be selected. If an alternate strain from the same genotype is not available, a strain different than those listed below shall be selected if available;
Tier 2 Bacillus anthracis Inclusivity Panel Species Strain
Notes
Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis Bacillus anthracis A Bacillus anthracis A Bacillus anthracis
Turkey 32
pXO1+, pXO2+; VNTR group A1b; genotype 23 pXO1+, pXO2+; VNTR group A3c; genotype 67 pXO1+, pXO2+; VNTR group A3d; genotype 68 pXO1+, pXO2+; VNTR group A3b; genotype 62 pXO1+, pXO2+; VNTR group B1; genotype 82 pXO1+, pXO2+; VNTR group A1a; genotype 7 pXO1+, pXO2+; VNTR group A2; genotype 29 pXO1+, pXO2+; VNTR group B2; genotype 80 pXO1+, pXO2+; VNTR group A4; genotype 69 pXO1+, pXO2-; VNTR group A3b; genotype 59, 61 pXO1+, pXO2-; VNTR group A3a; genotype 45
K3
Ohio ACB
Ames
BA1035 BA0018 PAK-1
RA3
SK-102
Sterne
V770-NP-1R Vollum 1B
pXO1+, pXO2+; VNTR group A4; genotype 77 Adapted from Official Methods of Analysis of AOAC INTERNATIONAL (2016) 20th Ed., AOAC INTERNATIONAL, Rockville, MD, USA, AOAC SMPR 2010.003, www.eoma.aoac.org , and Bacillus anthracis 2016.006 - Journal of AOAC International, Volume 99, Number 4, July-August 2016, pp. 1084-1089. A These two strains should not be included in the inclusivity panel if the product being tested only utilizes a pX02 assay, as these strains are expected to be pXO2 negative.
Bacillus anthracis Exclusivity - Tier 1
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