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characteristics and composition. Ideally, subsamples should be archived with associated 453 collection, processing, and characterization metadata in a searchable database.

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8.8.1 Air-Dried Soils 457 in a closed-lid container for up to five years. Before using the soil for experiments, the 458 soil should be well mixed. Soils should also be re-characterized prior to experiments 459 every 2 years. 463 immediately after collection or as soon as possible after that time. Until usage, the 464 samples should be stored at 4°C in a sealed container to limit microbial activity without 465 having a major impact on the microbial community composition. Studies show that 466 storage for more than 21 days at 4°C begins to impact some of the measurable microbial 467 activities (8). 471 studies. However, a manufactured soil is not recommended for full testing as it does not 472 recapitulate all of the properties of real soil. Manufactured soils are prepared using 473 defined recipes. As such, they do not include soil organisms that are an essential part of 474 the soil. Microorganisms impact nutrient cycling, soil structure, water retention and other 475 Soils that are processed by air drying and sieving can be stored at room temperature 8.8.2 Soils for Microbiological and Biochemical Applications Soils that are used specifically for their microbial community should be used either 468 469 470 8.9 Manufactured Soil For some applications, a manufactured soil may be appropriate for preliminary

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