SPADA Working Groups ~ April Meeting Book

AOAC INTERNATIONAL Stakeholder Panel on Agent Detection Assays Working Group Sessions - February 4, 2015 (Day 2)

I. Welcome and Introductions

Davenport opened the second day of SPADA proceedings and explained that since all were present from the previous day’s SPADA meeting, all were welcome to participate in the working groups. To sign up for specific working groups and stay engaged with them in the future, participants were encouraged to sign up through AOAC’s working group sign up form 10 .

II. VEE Working Group

Ostlund led the group in a discussion on the SMPR for VEE. The group agreed that the purpose of this SMPR will be the detection of agents if there is an aerosol release. There was a discussion on whether or not to develop one SMPR or two, one for confirmation and one for screening. The group agreed to limit the scope of the working group to one SMPR applicable to the detection of known human pathogenic strains of VEE, and possibly EEV and WEEV, in dry filters and or liquid samples from aerosol collectors, with the preferential method being a field-deployable assay that can detect specific viruses. A draft version of the Variola SMPR was used as a starting point and the group continued to progress through that template, adjusting it to fit VEE as needed. Definitions were reviewed and Ostlund took the action to provide an adequate definition of Venezuelan Equine Encephalitis/Encephalomyelitis Virus. AOAC took the action to incorporate the validation guidance and introduction paragraphs into the VEE SMPR. The group concluded its revisions with the first page of the template and agreed to continue progress by teleconference (attachment 1). Tallent led the group in a discussion on the SMPR for SEB. Applicability was agreed as specific detection of SEA, SEB, and SEC dry filters and/or liquid samples from aerosol collectors, with the preference of a field deployable assay. The first page of the template SMPR was reviewed and Tallent took the action to provide a definition of SEA-C for the definitions section. A lengthy discussion ensued regarding the Acceptable Minimum Detection Level (AMDL) for SEB. The group tentatively agreed to an AMDL of 25 ng / mL., but also agreed to revisit this issue once more data is available. Johns took the action to research this and bring it back to the next meeting of the SEB Working Group.

III. SEB Working Group

IV. Q-Fever Working Group

10 AOAC SPADA Working Group Sign Up: https://adobeformscentral.com/?f=Wwp88CLBMHw7QQ4Cr02L4g