SPADA at AOAC Annual Meeting 2023

‘RUMSFELDISM’ IN NGS APPLICATIONS

Donald Rumsfeld in a press briefing back in 2002 introduced the concept with respect to threats facing the country

Known Pathogen Unknown Target Usual suspects plus (SARS-CoV-2 variants) • Targeted AmpSeq • Isolate sequencing

Known Pathogen Known Target Usual suspects • Real time PCR • Targeted AmpSeq

Unknown Pathogen

Unknown Pathogen Known Target Newly

Unknown Target One that defies logic (Discovery) • MetaSeq • Isolate sequencing

emergent/engineered with usual attributes ( B. cereus var. anthracis ” CI) • Targeted AmpSeq (16S) • Isolate sequencing

Solving for complexity Posted on January 26, 2021 by Doug Belshaw

UNCLASSIFIED

10

AOAC Annual 2023