SPADA
Animal requirements
Sample
Toxin typing (A, B, E and F) (three dilutions)
Toxicity screening (three dilutions)
(46 mice)
Trypsin treated (3 dilutions, (2 mice per dilution = 6 mice
Non-trypsin treated (3 dilutions), (2 mice per dilution = 6 mice
Control (2 mice per Type of toxin) = 6 mice
3 dilution x 2 mice = 6 mice) (4 types x 6 mice = 24 mice)
Control (4 mice)
Alternate methods
DIG-ELISA
BioVeris M1M
DHS Biosensors
In vitro methods
E d ELISA n o- Bioassay (BBtech Inc)
Real time PCR
Lateral Flow Devices
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