SPADA

Background • Confirmation by isolation remains the gold  standard, in spite of aerosol hazard and low  infectious dose • Cultivation often includes antibiotics and  antibiotic dyes (thionin, basic fuchsin) to  counterselect against other microbes  • Select agent regulations complicate this  process!  Proposed rule changes out for  comment

Background Genome-based Brucella taxonomy Bohlin et al., 2010. BMC Evol. Biol. 10:249