SPADA
Real-time PCR assay for B. suis
• B. suis bv.1 (may also detect 2‐4, but results inconclusive with only one strain of each available) • 2 fg limit of detection (less than 1 genomic copy) • TaqMan assay developed on ABI 7000 and 7900HT Fast block (25μl rxn) • Detection in 15‐30 minutes
Roberto et al. 2009 Unpub (DHS)
Proposed Changes to FSAP Status • Removal of B. abortus , B. melitensis , and B. suis from HHS list of overlap agents because (1) mortality rate is low; (2) brucellosis is treatable with antibiotics; and (3) human‐to‐human transmission is “extremely rare” and zoonotic transfer is also rare (each point cites Lecaroz et al., 2006, J. Antimicrob. Chemotherap . 58:549‐ 546) – in spite of at least 3 human cases associated with feral swine hunting; dogs at risk • USDA/APHIS proposes to remove B. abortus and B. suis
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