SPDS 09052014

8/22/2014

Industry Needs for Chondroitin Sulfate

– Tests for Identity, Purity, Assay • Methods to verify the identity of the bulk material is  chondroitin sulfate • Methods to demonstrate that material does not  contain economic adulterants • Methods to quantify the amount of chondroitin sulfate in the material (possibly in the presence of  economic adulterants)

Identity Methods for Chondroitin Sulfate

– FTIR • polymeric nature of CS yields broad bands l h • simi arity to ot er GAGs • Limited utility – Optical Rotation (Specific Rotation) • CS is optically active and has characteristic specific  rotation • [α]= 20 0° to 30 0° (c=30 mg/mL) ‐ .  ‐ . • Not necessarily unique to CS

5