SPDS Aloe Vera Method Book

OFFICIAL DOCUMENT

Date Issued: Supersedes:

Page:

4 of 15

Procedure Title: Identification and quantitation of Aloe Vera Using 1 H NMR

N/A

Effective Date:

TBD

SOP #:

Revision #:

00

Prepared By: Department: Applies To:

Isaac Lee Quality Herbalife QC Laboratories

1.9

Sample Preparation (When only requires identification of aloe vera) 1.9.1 Powder Aloe Samples

1.9.1.1 For raw materials, weigh 7-14 mg of sample depending on concentration of material in a 12x75 mm test tube and add 0.7 ml of Nicotinamide solution. Vortex the solution and transfer it to a NMR tube. 1.9.1.2 For finished product, weigh about 20mg of sample in a 12 x 75 mm test tube, and add 0.7mL of Nicotinamide solution. Vortex the solution, and transfer it to a NMR tube. 1.9.1.3 Filter each dilution through a 0.45 μm filter prior to NMR analysis, if required. Liquid Aloe Samples 1.9.2.1 For raw materials, transfer about 0.7 mL of sample in a12x75 mm test tube, and add 0.7 mL of Nicotinamide solution. Vortex the solution and transfer 0.7mL of prepared sample to a NMR tube. 1.9.2.2 For finished product, transfer about 3.5 mL of sample in a 16x125 mm test tube, and add 0.7 mL of Nicotinamide Solution. Vortex and transfer 0.7 mL of prepared sample to a NMR tube. 1.9.2.3 Specifically for highly diluted aloe product, lyophilize 20-30 ml of the sample using a lyophilizer. Weigh about 40 mg of freeze dried sample in a12x75 mm tube, and add 0.7mL of Nicotinamide Solution. Vortex and transfer to a NMR tube. 1.9.2.4 Filter each dilution through a 0.45 μm filter prior to NMR analysis, if required. 1.10.1.1 For raw materials, weigh 7-14 mg of sample, depending on the materials concentration, 5mg of Nicotinamide, and 3mg of DSS in a glass vial. Add 0.7mL of D 2 O, and vortex the solution until the complete dissolution and transfer to a NMR tube. 1.10.1.2 For finished product, weigh 20mg of sample, 5mg of Nicotinamide, and 3mg of DSS in a glass vial. Add 0.7mL of D 2 O, and vortex the solution until the complete dissolution and transfer to a NMR tube. 1.10.1.3 Sonicate the solution if the complete dissolution is not reached using the vortex mixer alone. 1.10.1.4 If complete dissolution is not reached after the sonication, check the ingredients if the product contains any non-water soluble ingredients. Lower the sample amount if all ingredients should be dissolved in water. 1.10.2.1 For raw materials, weigh 0.7 mL of sample, 5mg of Nicotinamide, and 3mg of DSS in a glass vial. Add 0.3mL of D 2 O, and vortex the solution. Transfer 0.7mL of prepared sample to a NMR tube. 1.10.2.2 For finished product, lyophilize 20-30 ml of the sample using a lyophilizer. Weigh about 40 mg of freeze dried sample, 5mg of Nicotinamide, and 3mg of

1.9.2

1.10

Sample Preparation (When requires quantitation of aloe vera) 1.10.1 Powder Aloe Samples

1.10.2 Liquid Aloe Samples

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