SPDS Aloe Vera Method Book

OFFICIAL DOCUMENT

Date Issued: Supersedes:

Page:

5 of 15

Procedure Title: Identification and quantitation of Aloe Vera Using 1 H NMR

N/A

Effective Date:

TBD

SOP #:

Revision #:

00

Prepared By: Department: Applies To:

Isaac Lee Quality Herbalife QC Laboratories

DSS in a glass vial. Add 0.7mL of D 2

O and vortex the solution until the

complete dissolution. Transfer the solution to a NMR tube. 1.10.2.3 Sonicate the solution if the complete dissolution is not reached using the vortex mixer alone. 1.10.2.4 If complete dissolution is not reached after the sonication, check the ingredients if the product contains any non-water soluble ingredient. Lower the sample amount if all ingredients should be dissolved in water.

1.11

1 H NMR spectrums

DSS

Acetylated Glucomannan

Malic Acid

Glucose

Nicotinamide

Figure 1, Typical 1 H NMR Spectrum of aloe vera drink (finished produt)

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