SPDS Aloe Vera Method Book

OFFICIAL DOCUMENT

Date Issued: Supersedes:

Page:

9 of 15

Procedure Title: Identification and quantitation of Aloe Vera Using 1 H NMR

N/A

Effective Date:

TBD

SOP #:

Revision #:

00

Prepared By: Department: Applies To:

Isaac Lee Quality Herbalife QC Laboratories

Figure 5. Comparison of 1 H NMR spectra of Acacia gum and Aloe vera samples Locust bean gum 1 H NMR spectrum of Locust bean gum showed no Glucose (α- at 5.2 ppm and β- at 4.6 ppm), Malic acid (4.3 ppm), and Acetylated glucomannan (2.0-2.3 ppm) peaks (Figure 6). Therefore, this material will be confirmed as not an aloe vera material. In addition, the spectrum of Locust bean gum contains distinguishable peaks around at 1.06ppm region, so any aloe vera sample diluted with the locust bean gum is detectable (see figure 7). Therefore, this proton NMR ID method gives correct and reliable conclusion.

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