SPDS Aloe Vera Method Book

OFFICIAL DOCUMENT

Date Issued: Supersedes:

Page:

14 of 15

Procedure Title: Identification and quantitation of Aloe Vera Using 1 H NMR

N/A

Effective Date:

TBD

SOP #:

Revision #:

00

Prepared By: Department: Applies To:

Isaac Lee Quality Herbalife QC Laboratories

Figure 12. Comparison of 1 H NMR spectra of Maltodextrin and Aloe vera samples

1.14.3 Quantitation of aloeverose: Identify NMR peaks corresponding to acetyl groups of aloeverose in the Sample solution between 2.0 – 2.3ppm. Integrate peaks in this area. Calculate the percentage of Acetylated glucomannan in the sample solution using the following equation:

Result (Acetylated glucomannan %) = 3.06 x ( W N x I A

)/( W A

x I N

) x (Purity, %)

= weight of added Nicotinamide internal standard (mg)

W N

= integration area of acetylation methyls (multiple peaks in 2.0-2.3 ppm region) in aloeverose = sum of integration areas of the 4 aromatic CH peaks of the Nicotinamide standard = conversion factor of molecular weight and proton numbers for acetylated polysaccharide vs. Nicotinamide standard = weight of Aloe juice in Sample solution (mg)

I A

I N

W A

3.06

Purity, %

= Purity of Nicotinamide Standard

2.0

For Use Only By Herbalife International. This document contains confidential and proprietary information belonging to Herbalife International - It must not be reproduced or disclosed to others without prior written approval DEFINITIONS Acetylated glucomannan: Acetylated polysaccharides derived from aloe vera. It is also called Aloeverose or Acemannan.