SPDS Aloe Vera Method Book

2018 SPDS Method Submission Form ALO-03

Submission Date

2018-01-25 19:04:49

Submitter Name

Kan He

Submitter Email

kanh@herbalife.com

Organization

Herbalife International of America

Method Type

Aloe Vera (Quant or ID)

Method Name

Determination of Polysaccharides Molecular Weight in Aloe vera Fresh Leaf and Concentrated Juice Powder by SEC-RI

Method Author(s)

Yuehong Zhang, Zhichao Bao, Zhaoyang Xie, Kan He

Method Applicability Statement:

The method can be used to determine the relative molecular weight of polysaccharides in aloe vera using commercial available polysaccharides, pullulans, to establish a standard curve on SEC. The standard curve can be adjusted by using aloe polysaccharides with known molecular weights determined by MLAS measurement to obtain more accurate results. The method is suitable for polysaccharide molecular weight analysis by SEC, but without detectors for absolute MW determination, such light scattering detection.

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QCL Determination of Polysaccharides Molecular Weight in Aloe vera Fresh Leaf and Concentrated Juice Powder by SEC-RI.doc

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