SPDS Aloe Vera Method Book

Date Issued: Supersedes:

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1 of 7

Procedure Title: Determination of Organic Acid Contents in Aloe vera Fresh Leaf and Concentrated Juice Powder by HPLC Method

N/A

Effective Date:

QCL

SOP #:

Revision #:

Prepared By: Yuehong Zhang, Zhichao Bao Department: R&D Applies To: Herbalife International

CONTENTS Procedures Definitions References Forms/Chromatograms Ownership/Management Sign-off Revision History

SECTION 1.0 2.0 3.0 4.0 5.0 6.0

PURPOSE The purpose of this test method is to determine the amounts of organic acids in Aloe vera fresh leaf and concentrated juice powder by HPLC to establish the organic acid profile for Aloe vera identification.

REAGENTS 

DL-Malic acid, analytical standard, Sigma-Aldrich, Catalog# 94916 or equivalent  Acetic acid, analytical standard, Sigma-Aldrich, Catalog# 71251 or equivalent  Citric acid, analytical standard, Supelco, Catalog# 46933 or equivalent  DL-Isocitric acid trisodium salt hydrate, Sigma, Catalog# I1252 or equivalent  Isocitric acid lactone , Aldrich, Catalog# I16005 or equivalent  Oxalic acid dihydrate, Sinopharm Reagents, Catalog# 10014818 or equivalent  Tartaric acid, Sinopharm Reagents, Catalog# 10022018 or equivalent  Formic acid, Sinopharm Reagents, Catalog# 80065518 or equivalent  Lactic acid, Sinopharm Reagents, Catalog# 30108518 or equivalent  Succinic acid, Sinopharm Reagents, Catalog# 30171716 or equivalent  Fumaric acid, Aladdin, Catalog# F110741 or equivalent  Acetonitrile, HPLC grade  Phosphoric acid(85%-90%), HPLC grade  Ultrapure water or equivalent

EQUIPMENT 

Analytical Balance

 Disposable syringe filters, 25mm, 0.45 μm Hydrophilic PTFE membrane filter or equivalent  Disposable syringes (1mL)  Disposable plastic pipette  Vortex mixer  Sonicator  SCIENTZ-12N Lyophilizer  5-, 10-, 25-, 50-, 100, 250- and 500-mL Volumetric flasks  1.5-mL Glass HPLC vials HPLC  HPLC System: Waters 2695 Alliance Separations Module consisting of pump and autosampler or equivalent  Waters PDA detector or any variable wavelength UV detector or equivalent  HPLC Column: Phenomenex Luna C-18, 5 µm, 250 × 4.6 mm Part# 00G-4252-E0 and Thermo AcclaimTM OA, 5µm, 250 × 4.6 mm, product No. 062902 connected in series or equivalent  Guard Column: Phenomenex C-18, 4 x 3.0mm, Cat.# AJO-4287 or equivalent  HPLC chromatographic conditions: - Mobile Phase: 98% A = 0.2% Phosphoric Acid in Water 2% B = 100% Acetic Acid in Acetonitrile - Isocratic Condition

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