SPDS Aloe Vera Method Book

Date Issued: Supersedes:

Page:

5 of 7

Procedure Title: Determination of Organic Acid Contents in Aloe vera Fresh Leaf and Concentrated Juice Powder by HPLC Method

N/A

Effective Date:

QCL

SOP #:

Revision #:

Prepared By: Yuehong Zhang, Zhichao Bao Department: R&D Applies To: Herbalife International

isocitric acid lactone concentration is the difference of its standard concentration and the hydrolyzate, which amount is determined by isocitric acid standard.

Isocitric acid lactone solution actual conc. (μg/mL)

Hydrolyzate isocitric acid conc. (μg/mL)

= Isocitric acid lactone conc. (μg/mL)

—

Hydrolyzate isocitric acid conc. is obtained from the isocitric acid standard curve.

1.5.2

Raw Material / Finished Products Organic acid /Isocitric acid lactone (μg/mg) =

Spl. Conc. (μg/mL) x D.F. (mL) Spl. Wt. (mg)

Where

Spl. Conc. = sample concentration from the standard curve D.F. = dilution factor for sample

2.0

DEFINITIONS N/A REFERENCES N/A

3.0

FORMS / CHROMATOGRAMS 4.1

4.0

Chromatogram of isocitric acid standard.

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