SPDS Aloe Vera Method Book

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Procedure Title: Determination of Absolute Molecular Weight and Content of Polysaccharides in Aloe vera Fresh Leaf and Concentrated Juice Powder by SEC-MALS-RI

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Prepared By: Yuehong Zhang, Zhichao Bao Department: R&D Applies To: Herbalife International

The concentration of polysaccharides is determined by dividing resulting concentration obtained from RI detector over the injection mass calculated from ASTRA software.

Calculated mass (µg/100µl) x 10 x D.F.(mL) Sample Wt. (mg)

Polysaccharides (μg/mg) =

Calculated mass (µg/100µl) is known from Wyatt ASTRA 6.1.5.22 software D.F. = Dilution factor for sample

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DEFINITIONS N/A REFERENCES N/A

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FORMS / CHROMATOGRAMS 4.1 Chromatogram: Overlay light scattering (LS) and refractive index (RI) chromatograms of Aloe polysaccharides.

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OWNERSHIP/MANAGEMENT SIGN-OFF Approvals Refer to

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REVISION HISTORY Revision Date

Revised By

Summary of Items Revised New Test Method

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