SPDS Aloe Vera Method Book

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Standard Operating Procedures

Subject: Aloe Polysaccharides by O-Acetyl Department: Laboratory Original Date: 06/20/2016 Revision #: 1

Procedures #: CA17011

Approval Date:

Recovery Tablets 16.26% PS

mg/ml

added

Total

Assayed

ABS 540

g

ml 50 50 50

mg/ml Sample

mg/ml 0.0861 0.0861 0.0861

mg/ml 0.4149 0.4071 0.4214

mg/ml 0.4144 0.3960 0.4169

% Recovery

0.1011 0.0987 0.1031

2.02 1.97 2.06

0.3288 0.3210 0.3353

0.673 0.643 0.677

99.9 97.3 98.9 98.7

Average

Stdev % RSD

1.3 1.3

0.1065 0.1823 0.1878

50 50 50

2.13 3.65 3.76

0.3472 0.5943 0.6122

0.4303 0.4303 0.4303

0.7775 1.0246 1.0425

1.274 1.295 1.318

0.7836 1.0752 1.0943

100.8 104.9 105.0 103.6

Average

Stdev % RSD

2.4 2.3

Limit of Detection - 0.025 mg/ml Limit of Quantitation - 0.1 mg/ml mg/ml ABS 540 nm 0.05145 0.044 0.1029 0.110 0.2058 0.217 0.4116 0.437 0.6174 0.644 0.8232 0.848 1.029 1.029