SPDS EXPERT REVIEW PANEL

2018 SMPR ERPs - METHOD REVIEW FORM

Submission ID

4103501670324694861

Submission Date

2018-08-17 16:22:47

AOAC Expert Review Panel: Method Review

Reviewer Information

Name

Kan He

E-mail

kanh@herbalife.com

Organization

Herbalife Nutrition

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Aloe Polysaccharides by O-Acetyl

Title of Method

AOAC Candidate Method Number (e.g. ALN-01)

ALO-001

Applicable SMPR

SMPR2017.009

I. Summary of the Method

A. Summary of the Method

The method uses colorimetric quantitation of total acetyl group contents to determine the contents of acetylated polysaccharides in Aloe vera. The method contains two steps of chemical reactions: first the acetyl groups that attached at the polysaccharides of aloe were reacted with hydroxylamine to form acetohydroxamic acid, which was further reacted with ferric trichloride to form a ferric-acetohydroxamic acid complex and was measured at 540 nm wavelength.

II. Review of the Method Only: