SPDS EXPERT REVIEW PANEL

2018 SMPR ERPs - METHOD REVIEW FORM

Submission ID

4094755899471074196

Submission Date

2018-08-07 13:26:29

AOAC Expert Review Panel: Method Review

Reviewer Information

Name

John Szpylka

E-mail

john.szpylka@mxns.com

Organization

Merieux NutriSciences

Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:

SPDS SMPRs SPSFAM SMPRs

Method Review

Title of Method

Detection of flavokavins *A, B, C) in cultivars of kava (Piper methysticum) using high performance thin layer chromatography

AOAC Candidate Method Number (e.g. ALN-01)

KAV-003

Applicable SMPR

2018.006

I. Summary of the Method

A. Summary of the Method

Method detects the 6 major kavalactones and flavokavains A, B, and C in roots, stumps, basal stems, and extracts. Solid matrices are processed into 0.5mm screen powders, all samples dried and extracted overnight in acetone. After centrifugation the supernatants are analyzed using HP-TLC with hexane:dioxane as mobile phase.. Standards containing 6 major kavalactones and flavokavains A, B, and C at six concentrations are analyzed alongside samples.

II. Review of the Method Only: