SPDS EXPERT REVIEW PANEL
2018 SMPR ERPs - METHOD REVIEW FORM
Submission ID
4094755899471074196
Submission Date
2018-08-07 13:26:29
AOAC Expert Review Panel: Method Review
Reviewer Information
Name
John Szpylka
john.szpylka@mxns.com
Organization
Merieux NutriSciences
Remember, the main purpose of your review is to ensure the method conforms to the applicable SMPR. View and download SMPRs here:
SPDS SMPRs SPSFAM SMPRs
Method Review
Title of Method
Detection of flavokavins *A, B, C) in cultivars of kava (Piper methysticum) using high performance thin layer chromatography
AOAC Candidate Method Number (e.g. ALN-01)
KAV-003
Applicable SMPR
2018.006
I. Summary of the Method
A. Summary of the Method
Method detects the 6 major kavalactones and flavokavains A, B, and C in roots, stumps, basal stems, and extracts. Solid matrices are processed into 0.5mm screen powders, all samples dried and extracted overnight in acetone. After centrifugation the supernatants are analyzed using HP-TLC with hexane:dioxane as mobile phase.. Standards containing 6 major kavalactones and flavokavains A, B, and C at six concentrations are analyzed alongside samples.
II. Review of the Method Only:
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