SPDS ERP Review-May 2015

Liton Roy PhD

• Developed LCMS methods for quantitative and qualitative analysis of small molecules. • Developed and validated peptide fingerprinting, quantitative FTNIR, and quantitative SDS-PAGE methodologies. • Served as the subject matter expert for new product development and participated in related cross-functional teams. Driven analytical projects to support pre-clinical studies. • Resolved key method issues in QC by implementing advanced and reliable analytical methods resulting in 225,000 USD savings in one fiscal year. • Initiated third party scientific collaboration to address existing QC problems. University of Vermont, Burlington, VT (Graduate Teaching Assistant), 2006-2011 • Developed a new high throughput screening approach to select, identify, and characterize highly stable globular proteins. • Developed FPLC, HPLC, ESI-MS methodologies for the analysis of peptide libraries and bio- mimetic complexes. • Utilized nanospray mass spectrometry technique for ligand exchange kinetic study. • Designed metal directed coiled coil systems and analyzed electrostatic determinants of stability. • Routinely utilized GC, NMR, Mass spectrometry, and FTIR for small molecule characterizations. • Routinely utilized solid and solution phase peptide synthesis to produce peptide library (mixtures and discrete). • Led interdisciplinary research projects to completion. Michigan State University, East Lansing, MI (Research and Teaching Assistant) 2003-2005 • Developed HPLC and FPLC methodologies for enzymatic assays and protein purification. • Detailed mechanism of radical generation of SAM enzymes using ENDOR and EPR spectroscopies. • Synthesized isotopically labeled AdoMet synthetase by recombinant expression techniques. Bose Research Institute, Calcutta, India (Summer Intern) Summer 2002 • Characterized active sites of TIM barrel proteins using Rasmol, Protein Blast, and Homology screening. Technical Proficiencies and Research Skills • Over eight years of experience in LCMS (Agilent 6460 QQQ, ABI 4000 QTrap) and MALDI-TOF (Voyager DE-PRO). Skilled in ESI, APCI, Nanospray, and Direct infusion applications. Used H/D exchange analysis of small molecules. Skilled in bottom up mass spectrometric analysis of peptide libraries. Experienced with Masshunter and Analyst software. • Over ten years of experience in HPLC (RP, SEC, HIC, IEX) method development for small molecule quantitations, protein/peptide purifications, and separations. Experienced with Chemstation and Empower software. Executed HPLC/UPLC method transfer. Developed and validated assay, related substance, and stability indicating methods using DAD, MWD, FLD, and RI detectors. • Three years of experience in developing and validating Type I, II, III dissolution methods for tablets and softgels. • Expertise in GC-FID analysis of fatty acids and small molecules. Developed and validated GC- FID and GC-Head space methods under cGMP compliance. • Over ten years of experience in methodologies of UV/VIS, Fluorescence, CD, FTIR, and NMR spectroscopies. Experienced with Antaris FTNIR.