SPDS Lutein and Turmeric ERPs

7 . Any general comments about  the method?

The method is straightforward and clear for all three matrices. For dietary  supplement the procedure is simple Peaks are well separated in chromatogram ,  .   . This method is lack of separation of beta‐cryptoxanthin isomers and identification of  lutein and zeaxanthin.  Beta‐cryptoxanthin standard is included in the method but the purpose isn't clear.

Do you recommend this method be  adopted as a First Action and V.  Recommendation for the Method published in the Official Methods  of Analysis of AOAC  INTERNATIONAL?  Please specify  rationale.  

No. This method doesn't demonstrate the capability to separate beta‐cryptoxanthin  isomers as well as other carotnoids such as lutein and zeaxznthin Thus it doesn't ,   .  meet the requirement of SMPR. In additional, the single laboratory validation result  was lack of certain data such as reference material, system suitability, LOQ etc.