SPDS Lutein and Turmeric ERPs

Analytical Range:  0.2 to 4.2 mg/L in measured solutions but could not be  determined per AOAC.  LOQ:  0.2 mg/L  Accuracy / Recovery:  Recovery was 97%.  Could not be ascertained in the absence of cis‐isomer standards.  Precision:  RDS was  0.23%.  Reproducibility:  RSD <0.92% Conditions not provided to allow suitability to be replicated. —All instrument configuration; instrument details, including serial numbers and  acquisition information; and column details, including identification, were properly  recorded during laboratory operations. Equipment calibration, including signal  description and baseline noise determination, was performed before tests were  executed. This is the basis of a functional method. Several things could improve and expand  the method. It may be more applicable to measuring free lutein and zeaxanthin than  the esters. I would recommend that some things be adjusted before recommending  first action status.  Sample prep to address additional products. Assessment of the  composite‐specific absorbance. Inclusion of standards and beta‐cryptoxanthin.

Performance Characteristics

System Suitability

Do you recommend this method be  adopted as a First Action and  published in the Official Methods  of Analysis of AOAC  INTERNATIONAL?  Please specify  rationale.   Recommendation for the Method