SPDS Lutein and Turmeric ERPs

5. Based on the supporting  information, what are the  pros/strenghts of the method?

The method is straightforward and relatively simple.  It has been tested on a number  of matrices (as suggested in Table 3 of SMPR) and in the presence of numerous  other botanicals (including 2 from Table 2 of SMPR).  The validation is solid and the  method performance meets nearly every aspect of the SMPR.

6. Based on the supporting  information, what are the cons  /weaknesses of the method?

Would prefer an internal standard approach to the external standard approach  presented here.

LC method has a very minor gradient step (28‐30%); is this necessary?  Seems that  an isocratic method with a wash step might be more robust. Not all dietary ingredients from Table 2 in SMPR were included in testing (e.g.,  cayenne, carotenoids, Ca2+).  Would like to see demonstration of method on  products including these to meet SMPR.

7 . Any general comments about  the method? V.  Recommendation for the Method Do you recommend this method be  adopted as a First Action and  published in the Official Methods  of Analysis of AOAC  INTERNATIONAL?  Please specify  rationale.  

Very strong method, well validated!

Yes, I would support this method for First Action status, but would recommend  additional work on investigation of remaining dietary ingredients listed in SMPR  before MLT (and also include a mixed product containing these in the MLT).