SPDS Lutein and Turmeric ERPs

General Information

Reviewer Name:

Elizabeth Mudge emudge@bcit.ca

Email:

Organization:

BCIT

Method Reviewed:

TUR‐02

Method Title:

Quantitation of Curcuminoids

Applicable SMPR

2016.003

I.  Summary

Summary of Method:

This method is to quantify curcuminoids (curcumin, demethoxycurcumin and bis‐ demethoxycurcumin) in turmeric roots and finished products using HPLC with  detection at 425 nm. Yes ‐ this method is applicable to quantitation of the three major curcuminoids in  turmeric roots and finished products

1.  Does the Applicability of the  Method Support the Applicability  of the SMPR?  If not, please explain  what is missing. II.  Review of the Method Only 2.  Does the analytical technique(s)  used in the method meet the  SMPR?  If not, please specify how it  differs from what is stated in the  SMPR. 3.  Are the definitions specified in  the SMPR used and applied  appropriatly in the method?  If no,  please indicate how the terms are  used. 4.  Does the method, as written,  contain all appropriate precautions  and warnings related to the  method's regaents, components,  instrumentation, or method steps  that may be hazardous?  If no,  please suggest wording or  option(s). 1.  Are the definitions specified in  the SMPR used and applied  appropriately in the supporting  documentation (manuscripts,  method studies, etc…)?  If not,  please explain the differences and  if the method is impacted by the  difference.   2.  Is there information  demonstrating that the method  meets the SMPR Method  Performance Requirements using  the Reference Materials stated in  the SMPR?   If not,  then specify  what is missing and how this  impacts demonstration of  performance of the method.  

Yes.

Yes.

Yes ‐ most of this method does not require significant safety warnings

III.  Review of Information in Support of the Method

For the most part, yes ‐ with the exception of repeatability. The studies performed  here specify 6 replicates, but in this case these are actually triplicate samples with  duplicate injections, treated as six replicates. This data is therefore somewhat biased  to improve the precision of the method because replicate injections of the same  sample will improve repeatability compared with six actual replicate samples which  take into account the entire sample preparation.

No reference materials were used in this study report.