SPDS Lutein and Turmeric ERPs

7 . Any general comments about  the method?

The selection of extraction solvent seemed to be based more on the precision of the  replicates rather than on analyte recovery. When looking at the error, the error of  the methanol is slightly larger, but there is a significant difference between the  quantity of curcumin and DMC extracted with methanol compared with the 80%  methanol. Without any recovery data in this study, it appears that the accuracy of  this method may be insufficient. There were also re‐extraction repetitive dilution  study, but without descriptions of the actual methodologies, it is difficult to  understand how this graph explains that only 1 extraction is required. No. The method appears to be missing key information including method  recovery/accuracy. The repeatability data was calculated using duplicate injections  as replicates and the selection of extraction solvent appears to impact the recovery  of the curcuminoids.

Do you recommend this method be  adopted as a First Action and  published in the Official Methods  of Analysis of AOAC  INTERNATIONAL?  Please specify  rationale.   V.  Recommendation for the Method