SPDS Lutein and Turmeric ERPs

6.  Based on the supporting  information, what are the cons  /weaknesses of the method?

No tinctures or liquids were tested.  Would like to see demonstration of method on  these types of products to meet SMPR. Method was not tested in the presence of carotenoids, piper nigrum, or Ca. Would  like to see demonstration of method on products including these to meet SMPR.

Would prefer an internal standard approach to the external standard approach  presented here.

No recovery data ‐ would need recovery data to meet SMPR.

Several concerns about extraction optimization.  100% methanol gives 10%+ better  extraction efficiency, yet 80:20 methanol:water was selected.  Also, additional  curcuminoids are being extracted in 2nd‐4th extractions. This could be because  80:20 methanol:water is not as effective as 100% methanol, but would like to see  the same study with 100% methanol.

7 . Any general comments about  the method? V.  Recommendation for the Method Do you recommend this method be  adopted as a First Action and  published in the Official Methods  of Analysis of AOAC  INTERNATIONAL?  Please specify  rationale.  

No

Not at this time. I think significant work is needed on the extraction protocol and  more products need to be explored before this method could be First Action.