SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 2000 mg / 100 ml : Trl 2

Vail #

: 37

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP008.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 6:53:38 AM : 1/23/2007 3:16:49 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP008.lcd

mV

1Det.A Ch1

500

3

1

4

250

5

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1352797 1130441 3101015 1556432 1097660 349933 8588278

Height

Area %

Name

1 2 3 4 5 6 Total

15.561 19.717 20.387 21.390 22.247 25.528

239294 162804 439045 257206 197192 62551 1358092

15.752 13.163 36.108 18.123 12.781 4.075 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP008.lcd