SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 1000 mg / 100 ml : Trl 1

Vail #

: 45

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II003.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 3:16:50 PM : 1/23/2007 2:58:55 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II003.lcd

mV

300

1Det.A Ch1

3

200

1

4

5

100

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.372 19.526 20.216 21.253 22.119 25.444

677881 560865 1542242 769019 557605 171769 4279382

118436 80925 215590 125023 97740 30570 668285

15.841 13.106 36.039 17.970 13.030 4.014 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II003.lcd