SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera : WS / 06 lot 10

Sample ID

: 2000 mg / 100 ml : Trl 1

Vail #

: 49

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Rep-RP-II007.lcd : Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/16/2007 6:22:05 PM : 1/23/2007 3:04:28 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II007.lcd

mV

1Det.A Ch1

500

3

400

300

1

4

5

200

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1333648 1102054 3036695 1511759 1120023 338649 8442826

Height

Area %

Name

1 2 3 4 5 6 Total

15.390 19.562 20.248 21.284 22.149 25.476

234986 158403 424260 245224 193935 60102 1316911

15.796 13.053 35.968 17.906 13.266 4.011 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II007.lcd