SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - 06 LOT 08

Vail #

: 18

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Quan005.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 11:04:04 AM : 1/23/2007 2:51:10 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan005.lcd

mV

1Det.A Ch1

400

3

300

4

1

200

5

2

100

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 1057665 918398 2591905 1290000 929280 287702 7074950

Height

Area %

Name

1 2 3 4 5 6 Total

15.536 19.718 20.387 21.394 22.251 25.531

187666 131432 364794 212280 167586 51262 1115020

14.949 12.981 36.635 18.233 13.135 4.066 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan005.lcd