SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - 05 LOT 20

Vail #

: 14

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Quan001.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 8:01:07 AM : 1/23/2007 2:45:58 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan001.lcd

mV

1Det.A Ch1

3

100

5

4

50

1

2

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.525 19.708 20.378 21.383 22.242 25.522

205437 168059 812277 263678 246190 68520 1764161

37048 24112 113984 45550 44160 12524 277378

11.645 9.526 46.043 14.946 13.955 3.884 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan001.lcd