SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: RM - ERH - 46

Vail #

: 21

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Quan008.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 1:21:18 PM : 1/23/2007 2:54:40 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan008.lcd

mV

1Det.A Ch1

3

150

1

100

4

5

2

50

6

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.547 19.698 20.372 21.377 22.239 25.523

491157 473414 1250895 410796 384409 102080 3112751

87210 67676 178900 70915 66596 19017 490313

15.779 15.209 40.186 13.197 12.349 3.279 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan008.lcd