SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: WS - 06 LOT 10

Vail #

: 23

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: SR002.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 2:52:46 PM : 1/23/2007 3:23:04 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SR002.lcd

1Det.A Ch1

400

3

4

300

5

6

1

200

2

100

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm

Detector A Ch1 227nm Peak # Ret. Time

Area 1069212 927543 2707641 1846124 1500314 1335664 9386498

Height

Area %

Name

1 2 3 4 5 6 Total

15.515 19.695 20.367 21.381 22.244 25.531

186204 132363 384787 308955 261841 234969 1509118

11.391 9.882 28.846 19.668 15.984 14.230 100.000

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SR002.lcd