SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania somnifera ext

Sample ID

: RM - ERH - 46

Vail #

: 29

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: SSR004.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/15/2007 7:27:15 PM : 1/23/2007 3:30:32 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SSR004.lcd

500

1Det.A Ch1

3

4

400

5

300

6

200

1

100

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.431 19.614 20.297 21.327 22.196 25.516

828923 432974

140122 61422 480548 453987 345301 254464 1735844

7.668 4.005

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

3319438 2686515 2078342 1464553 10810745

30.705 24.850 19.225 13.547 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\SSR004.lcd