SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withanolides : std mix

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\SM-01-Rep2

Vial No

: 10001

Injection Volume

: 20

Date & Time

: 4/3/07 8:30:25 PM

0.8

0.6

0.4

Volts

23.883

23.008

22.017

26.975

0.2

17.208

21.458

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.208 21.458 22.017 23.008 23.883 26.975

Withanoside IV Withanoside V Withaferin A

569898 272130 1797785 1787246 1842576 1513567

7.32 3.50

23.10 22.96 23.67 19.45

Withastramonolide

Withanolide A Withanolide B

Totals

7783202

100.00