SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 06 lot 08

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S3-Rep1

Vial No

: 10004

Injection Volume

: 20

Date & Time

: 4/4/07 4:55:00 AM

0.4

22.017

0.3

17.217

23.008

0.2

Volts

23.900

21.492

0.1

26.975

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.492 22.017 23.008 23.900 26.975

Withanoside IV Withanoside V Withaferin A

1195002 802479 2091436 1179907 1073657 261990

18.09 12.15 31.67 17.87 16.26

Withastramonolide

Withanolide A Withanolide B

3.97

Totals

6604471

100.00