SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : WS - 05 lot 20

: C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S1-Rep1

Vial No

: 10002

Injection Volume

: 20

Date & Time

: 4/4/07 9:30:05 AM

0.20

22.075

0.15

0.10

Volts

17.225

23.917

23.050

0.05

21.417

27.042

0.00

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.225 21.417 22.075 23.050 23.917 27.042

Withanoside IV Withanoside V Withaferin A

298076 187974 952512 311700 330290

13.93

8.79

44.52 14.57 15.44

Withastramonolide

Withanolide A Withanolide B

58776

2.75

Totals

2139328

100.00