SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : RD - 1045 : C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S5-Rep1

Vial No

: 10006

Injection Volume

: 20

Date & Time

: 4/4/07 2:05:10 PM

0.5

0.4

0.3

22.058

Volts

17.217

0.2

23.033

23.908

21.408

0.1

27.025

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.408 22.058 23.033 23.908 27.025

WIthanoside IV Withanoside V

891560 482933 1539138 572491 793283 168573

20.04 10.86 34.60 12.87 17.83

Withaferin A

Withastramonolide

Withanolide A Withanolide B

3.79

Totals

4447978

100.00