SPDS SET 2: ASH-01

QUALITY CONTROL DEPARTMENT

Name of Sample Method Name Data File Name

: Withania somnifera : RD - 1045 : C:\Methods\WITHANIA.met

: D:\LC10-ATvp\2007\Method validation\Apr 2007\020407\S5-Rep3

Vial No

: 10006

Injection Volume

: 20

Date & Time

: 4/4/07 3:36:53 PM

0.6

0.5

0.4

0.3

22.067

Volts

17.217

0.2

23.042

23.908

21.400

0.1

27.042

0.0

0

5

10

15

20

25

30

35

40

45

Minutes

Detector A - 1 (227nm)

Pk # Retention Time Name

Area Area Percent

1 2 3 4 5 6

17.217 21.400 22.067 23.042 23.908 27.042

WIthanoside IV Withanoside V

883242 487860 1557209 585455 802542 170641

19.68 10.87 34.71 13.05 17.89

Withaferin A

Withastramonolide

Withanolide A Withanolide B

3.80

Totals

4486949

100.00