SPDS SET 2: ASH-01
3.0] Materials and Method:
3.1) Solvents:
Solvent Name
Grade HPLC
Product No.
Batch No. R014F06 R246A07 R189A07 R180M06
Supplier Rankem
Acetonitrile
A0755
Methanol
HPLC
M0275
Rankem
Water
Ultra pure Grade
From Sartorius water purification system.
3.2) Chemicals and their details
3.2.1) Reference Standard: Withanoside IV, Withanoside V, Withaferin A, 12-Deoxy Withastramonolide,
Withanolide A, Withanolide B Reference standard with % purity >90 %.
Reference standard
Lot No. T6B107
% Purity 90% w/w
Supplier
Withanoside IV
Withanoside V
T5A096
90% w/w
Withaferin A
T7A047
99% w/w
Natural Remedies Private Limited, Bangalore
12- Deoxy withastramonolide
T7A099
95% w/w
Withanolide A
T6K051
99% w/w
Withanolide B
T6A088
99% w/w
3.2.2) Other sources of reference standard: M/s. Chromadex and M/s. Extrasynthese
3.2.3) Sample and their details: Sample
Standardized to
Batch No RD/1162
Raw material
Total withanolides >0.2% by HPLC
Raw material
Total withanolides >0.2% by HPLC
ERH-046
Water extract
Total withanolides >0.15% by HPLC
WS–05Lot20
Hydro-alcoholic extract
Total withanolides >1.0% by HPLC
WS–05Lot21
Methanolic extract
Total withanolides >1.5% by HPLC
RD–1045
Methanolic extract
Total withanolides >2.5% by HPLC
WS–06Lot08
Methanolic extract
Total withanolides >2.5% by HPLC
WS–06Lot10
Methanolic extract
Total withanolides >2.5% by HPLC
RD–1170
QC Check sample: The QC check sample is the reference extract of Withania somnifera extract.
It is prepared out of botanically authenticated Withania somnifera and confirmed for the presence
of Withanoside IV, Withanoside V, Withaferin A, 12-Deoxy withastramonolide, Withanolide A and
Withanolide B using the respective reference standards.
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3DJH RI
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