SPDS SET 2: ASH-01

PEAK AREA OF WITHANOSIDE V

Table-12

Withanoside V (mcg/ml)

Different Concentrations

Dilution -6 (18.76)

Dilution -5 (37.52)

Dilution -4 (75.04)

Dilution -3 (150.08)

Dilution -2 (300.15)

Dilution -1 (600.30)

S. solution (1200.60)

Replicate – 1

151294

298008

586819

1146136

2238822

4334899

8731747

Replicate –2

151121

297953

585559

1146843

2237319

4327804

8721728

Replicate –3

151156

298036

585906

1141122

2243282

4330341

8719358

Replicate –4

151277

297352

581818

1152990

2237337

4338646

8718285

Replicate – 5

150985

297517

584964

1151745

2248262

4345473

8714428

Mean

151167

297773

585013

1147767

2241004

4335433

8721109

7222.11

7263.96

Response factor Area / concentration Mean (Dilution 2 to 6) RSD (Dilution 2 to 6)

8058.19

7936.65

7796.28

7647.96

7466.28

7781.072

2.68

LINEARITY GRAPH PEAK AREA Vs CONCENTRATION

10000000

7500000

5000000

Area

2500000

0 250 500 750 1000 1250 1500 0 Concentration of Withanoside V (mcg/ml)

Best-fit values Slope

7225 ± 10.77 37920 ± 5644 -5.249 0.0001384 7203 to 7247 26430 to 49410 -8.152 to -2.352

Y-intercept when X=0.0 X-intercept when Y=0.0 1/slope 95% Confidence Intervals Slope Y-intercept when X=0.0 X-intercept when Y=0.0

Goodness of Fit r² Sy.x Is slope significantly non-zero? F DFn, DFd P value Deviation from zero? Data Number of X values Maximum number of Y replicates Total number of values Number of missing values

0.9999 25390

450000 1.000, 33.00 < 0.0001 Significant

7 5 35 0

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