SPDS SET 2: ASH-01

PEAK AREA OF WITHANOLIDE A

Table-15

Withanolide A (mcg/ml)

Different Concentrations

Dilution -6 (21.59)

Dilution -5 (43.19)

Dilution -4 (86.38)

Dilution -3 (172.76)

Dilution -2 (345.51)

Dilution -1 (691.02)

S. solution (1382.04)

Replicate – 1

551317

1084798

2130939

4137256

7955602

13463661

13878628

Replicate –2

550721

1084624

2130222

4140860

7957220

13489137

13809664

Replicate –3

550930

1084955

2130126

4118314

7958121

13484780

13766317

Replicate –4

550882

1084918

2112327

4144205

7963123

13502916

13721170

Replicate – 5

552430

1082926

2131439

4149692

7973789

13491381

13664575

Mean

551256

1084444

2127011

4138065

7961571

13486375

13768071

23042.95

19516.62

9962.14

Response factor Area / concentration Mean (Dilution 3 to 6) RSD (Dilution 3 to 6)

25527.76

25109.41

24624.59

23953.38

24803.79

2.36

LINEARITY GRAPH PEAK AREA Vs CONCENTRATION

15000000

10000000

Area

5000000

0 100 200 300 400 500 600 700 800 0 Concentration of Withanolide A (mcg/ml)

Best-fit values Slope

19430 ± 335.7 486700 ± 109300 -25.05 0.00005148 18740 to 20110 262700 to 710600 -45.92 to -4.814

Y-intercept when X=0.0 X-intercept when Y=0.0 1/slope 95% Confidence Intervals Slope Y-intercept when X=0.0 X-intercept when Y=0.0

Goodness of Fit r² Sy.x Is slope significantly non-zero? F DFn, DFd P value Deviation from zero? Data Number of X values Maximum number of Y replicates Total number of values Number of missing values

0.9917 430000

3348 1.000, 28.00 < 0.0001 Significant

6 5 30 0

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