SPDS SET 2: ASH-01

Table-48: B. No.RD/1045 Replicate Withanoside IV

Withanoside V

Withaferin A

12-Deoxy withastramonolide

Withanolide A

Withanolide B

Sum

0.603

0.361

0.399

0.247

0.130

0.042

1.782

Replicate - 1

0.603

0.366

0.400

0.247

0.130

0.042

1.788

Replicate - 2

0.610

0.365

0.407

0.251

0.132

0.043

1.809

Replicate - 3

0.606

0.364

0.402

0.248

0.131

0.042

1.793

Mean

0.68

0.79

1.09

0.95

1.09

0.97

0.80

RSD

Table-49: B. No.RD/1162 Replicate Withanoside IV

Withanoside V

Withaferin A

12-Deoxy withastramonolide

Withanolide A

Withanolide B

Sum

0.150

0.089

0.099

0.021

0.025

0.004

0.388

Replicate - 1

0.153

0.090

0.101

0.021

0.025

0.004

0.395

Replicate - 2

0.152

0.090

0.100

0.020

0.024

0.004

0.390

Replicate - 3

0.152

0.090

0.100

0.021

0.025

0.004

0.391

Mean

1.06

0.74

0.75

1.0

1.06

1.24

0.86

RSD

Table-50: B. No.ERH/46 Replicate Withanoside IV

Withanoside V

Withaferin A

12-Deoxy withastramonolide

Withanolide A

Withanolide B

Sum

0.185

0.149

0.146

0.041

0.041

0.010

0.573

Replicate - 1

0.182

0.146

0.144

0.041

0.041

0.010

0.563

Replicate - 2

0.186

0.148

0.146

0.042

0.042

0.010

0.574

Replicate - 3

0.184

0.148

0.145

0.041

0.041

0.010

0.570

Mean

1.26

0.88

1.0

1.09

1.34

1.46

1.07

RSD

4.4.2) Conclusion: 1) Relative standard deviation for the mean values of assay of Withanoside IV in the samples is <2.5% except WS/05Lot20 where it is 4.5% because the contents are very low. 2) Relative standard deviation for the mean values of assay of Withanoside V in sample is

<2.5% except WS/05Lot20 where it is 3.92% because the contents are very low.

3) Relative standard deviation for the mean values of assay of Withaferin A in sample is <2.5%

except WS/05Lot20 where it is 4.68% because the contents are very low. 4) Relative standard deviation for the mean values of assay of 12-Deoxy withastramonolide in <2.5% except WS/05Lot20 where it is 5.13% because the contents are very low. 5) Relative standard deviation for the mean values of assay of Withanolide A in sample is <2.5% except WS/05Lot20 where it is 5.46% because the contents are very low. 6) Relative standard deviation for the mean values of assay of Withanolide B in sample is

<2.5% except WS/05Lot20 where it is 3.68% because the contents are very low.

Enclosure-7: Standard chromatograms (3 Pages) Enclosure-8: Sample chromatograms (24 pages).

1$785$/ 5(0(',(6

3DJH RI