SPDS SET 2: ASH-01

Experiment-2 (Condition - 2) b) Standard / sample preparation: Refer 4.3.11

Table-66:

Batch No.

Withanoside IV

Withanoside V

Withaferin A

12-Deoxy withastra monolide

Withanolide A

Withanolide B

Sum

WS/06Lot08

1.071

0.629

0.608

0.304

0.290

0.071

2.974

WS/06Lot10

1.047

0.601

0.561

0.282

0.258

0.067

2.817

WS/05Lot20

0.080

0.044

0.083

0.024

0.027

0.0005

0.263

WS/05Lot21

0.538

0.298

0.415

0.130

0.128

0.027

1.537

RD/1170

0.995

0.599

0.740

0.257

0.348

0.080

3.020

RD/1045

0.744

0.357

0.422

0.140

0.201

0.043

1.906

RD/1162

0.162

0.083

0.090

0.022

0.025

0.0004

0.386

ERH-46

0.213

0.147

0.141

0.046

0.403

0.011

0.601

Table-67:

Batch No.

Condition

Sum

WS/06Lot08

1 2

2.974 2.802

RSD

2.977839

WS/06Lot10

1 2

2.817 2.681

RSD

2.473627

WS/05Lot20

1 2

0.263 0.241

RSD

4.365079

WS/05Lot21

1 2

1.537 1.453

RSD

2.809365

RD/1170

1 2

3.020 2.779

RSD

4.155889

RD/1045

1 2

1.906 1.793

RSD

3.05488

RD/1162

1 2

0.386 0.391

RSD

0.643501

ERH-46

1 2

0.601 0.570

RSD

2.64731

4.6.5) Conclusion: Relative standard deviations for same batch sample when analysed in two

different instruments found to be < 6.0% indicating the method is very rugged.

Enclosure 12: Standard and sample chromatograms (27 pages)

1$785$/ 5(0(',(6

3DJH RI