SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : I

Vail #

: 12

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin026.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 7:40:57 PM : 1/23/2007 2:23:54 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin026.lcd

mV

1Det.A Ch1

4

5

6

3

2000

1500

1000

1

2

500

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 4815460 4334899 14666731 14809446 13463661 12728719 64818916

Height

Area %

Name

1 2 3 4 5 6 Total

15.732 19.926 20.563 21.542 22.391 25.639

764233 615202 2039992 2177743 2076735 2047502 9721408

7.429 6.688

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

22.627 22.847 20.771 19.637 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin026.lcd