SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : II

Vail #

: 11

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin022.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 4:37:50 PM : 1/23/2007 2:19:11 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin022.lcd

mV

1Det.A Ch1

1500

4

6

5

3

1000

500

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 2493747 2237319 8322031 9138372 7957220 7947620 38096308

Height

Area %

Name

1 2 3 4 5 6 Total

15.500 19.689 20.349 21.366 22.225 25.514

395202 319880 1208671 1501621 1300693 1358431 6084499

6.546 5.873

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.845 23.988 20.887 20.862 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin022.lcd