SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : II

Vail #

: 11

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin024.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 6:09:22 PM : 1/23/2007 2:20:56 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin024.lcd

mV

1Det.A Ch1

1500

4

6

5

3

1000

500

1

2

0

7

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area 2492809 2237337 8328440 9151223 7963123 7953428 2416 38128776

Height

Area %

Name

1 2 3 4 5 6 7 Total

15.497 19.697 20.361 21.382 22.247 25.551 44.827

393646 316713 1206071 1497652 1302715 1354058 116 6070972

6.538 5.868

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.843 24.001 20.885 20.859 0.006 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin024.lcd