SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : IV

Vail #

: 9

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin013.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 9:45:52 AM : 1/23/2007 2:02:54 AM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin013.lcd

mV

1Det.A Ch1

750

500

4

6

5

3

250

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.512 19.694 20.362 21.380 22.245 25.538

651630 585906

103712 83388 327394 414714 352980 374025 1656213

6.374 5.731

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

2228288 2471386 2130126 2156648 10223983

21.795 24.172 20.835 21.094 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin013.lcd