SPDS SET 2: ASH-01

NATURAL REMEDIES PVT LIMITED QUALITY CONTROL DEPARTMENT

Acquired by Sample Name

: Admin

: Withania : std mix

Sample ID

: Dilution : VI

Vail #

: 7

Injection Volume Data File Name Method File Name Batch File Name Report File Name Data Acquired Data Processed

: 20 uL

: Lin001.lcd

: Withaina.met.lcm : Batch 01.lcb : Calibration.lcr

: 1/14/2007 12:36:53 AM : 1/20/2007 4:58:40 PM

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin001.lcd

mV

1Det.A Ch1

750

500

250

4

6

5

3

1

2

0

0

5

10

15

20

25

30

35

40

45

min

Det.A Ch1/227nm 1

Detector A Ch1 227nm Peak # Ret. Time

Area

Height

Area %

Name

1 2 3 4 5 6 Total

15.466 19.651 20.320 21.351 22.219 25.517

166047 151294 573810 635487 551317 553596 2631550

26493 21445 84100 106310 91243 96384 425976

6.310 5.749

Withanoside IV Withanoside V Withaferin A 12- Deoxy withastramonolide

21.805 24.149 20.950 21.037 100.000

Withanollide A Withanolide B

D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Lin001.lcd